Histone-modifying enzymes, histone modifications and histone chaperones in nucleosome assembly: Lessons learned from Rtt109 histone acetyltransferases

Histone Antibodies Histone Purification Recombinant Histones Histone Modifying Enzymes Histone Modification ELISAs Histone Demethylase Assay Histone Peptide Arrays Histone Binding Assay HAT/HDAC Assays Chromatin Assembly

Active Motif's unique portfolio of histone technologies provides researchers with a complete solution for the analysis of histones and their post-translational modifications, beginning with histone purification and continuing through to chromatin assembly. Let Active Motif's antibodies, enzymes and modification-specific assays simplify your histone analysis.

SnapShot: Histone-Modifying Enzymes

P-98: Effect of Mouse Embryo Vitrification on Histone Modifications

Background: Vitrification has been usually used as an assisted reproductive technology in animals and humans. This method needs high concentrations of cryoprotectants that can be toxic with high cooling degrees. Then, vitrification could be change histone modifications such as methylation and acetylation can performance as regulatory controls of gene transcription. So, the purpose of the presen...

Histone Chaperones: Functions beyond Nucleosome Deposition

Histones, the structural unit of chromatin, must be assembled/dissembled to preserve or change chromatin organization in accordance to cellular needs. Initially, function of histone chaperones was thought to be only “histone carriers/vehicles”, but now with accumulating evidences they are known to be the key actors of histone metabolism. With this outburst of knowledge, histone chaperones are n...

Histone chaperones regulate histone exchange during transcription.

Transcription by RNA polymerase II is accompanied by dynamic changes in chromatin, including the eviction/deposition of nucleosomes or the covalent modification of histone subunits. This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, using a dual histone expression system. Th...